BSI Logo

Laboratory for Cell Function Dynamics

BSI Building

lab head: Atsushi Miyawaki, M.D., Ph.D.

    Home >Sample Request (MTA) >Fucci

Fucci

We are writing to relay our concern about the inaccurate terminology of the cell-cycle probe, Fucci. Whereas an increasing number of papers report cell cycle dynamics by use of the Fucci technology, the two Fucci probes, mKO2-hCdt1(30/120) and mAG-hGem(1/110), are often called mKO2-Cdt1 and mAG-Geminin, respectively. Likewise, the two Fucci2 probes are often called mCherry-Cdt1 and mVenus-Geminin. However, this terminology would mislead readers into believing that the Fucci probe contains the entire protein of Cdt1 or Geminin. In fact, more and more researchers are asking us whether such probes perturb the cell cycle regulation of recipient cells. In our original study of Fucci (Sakaue-Sawano et al. 2008), we made considerable effort to extract the regulatory domains (ubiquitination domains) from Cdt1 and Geminin; after performing long-term time-lapse imaging of numerous constructs, we demonstrated that amino acid residues 30-120 of human Cdt1 (hCdt1(30/120)) and amino acid residues 1-110 of human Geminin (hGem(1/110)) are both necessary and sufficient for the purpose. Thus, we would like to request that you use correct terminology listed on the worksheet below in your forthcoming publications.

Thank you for your understanding.
                                                                                                               Atsushi Miyawaki

Material
 
Plasmids
Please complete WORD the worksheet and send it to Ms. Sakurai
at sakurai-h"at"brain.riken.jp
Link
Material
 
CSII-EF-MCS
pCAG-HIVgp
pCMV-VSV-G-RSV-Rev

RIKEN Tsukuba
Bio Resource Center
Subteam for Manipulation of Cell Fate

Maps are also available from the website

Cell lines

HeLa.S-Fucci  (RCB 2812)
NMuMG-Fucci (RCB 2813)
COS-Fucci (RCB 2814)

published in
Cell 2008

RIKEN Tsukuba
Bio Resource Center
Cell Bank

HeLa.S-Fucci2 (RCB 2867)
NMuMG-Fucci2 (RCB 2868)
BMC CB
2011
Mouse lines
FucciS/G2/M-#504 (RBRC 02706)
FucciG1-#596 (RBRC 02707)
Cell 2008
FucciS/G2/M-#492 (RBRC 02705)
FucciG1-#639 (RBRC 02709)
PNAS 2010
FucciS/G2/M-#474 (RBRC 02704)
FucciG1-#610 (RBRC 02708)
Development
2013b
Fucci2aR(RBRC 06511)

Cell Cycle
2014

R26p-Fucci2
R26R-Fucci2
R26-Fucci2

Development
2013a

RIKEN Kobe
Center for Developmental Biology, LARGE
Contact Info: mutant"at"cdb.riken.jp
Zebrafish lines

EF1α:mKO2-zCdt1(1/190)rw0405b
EF1α:mKO2-zCdt1(1/190)rw0405d
EF1α:mAG-zGem(1/100)rw0410h
EF1α:mAG-hGem(1/60)rw0412a

PNAS 2009

National BioResource Project Zebrafish

Drosophila lines

Mol Cell Biol 2011
Development
2011

Drosophila Gnetic Resource Center
Fucci related products Life Technologies Co.
Amalgaam Inc.
Clontech Laboratories Inc.


Accession no. of DDBJ/EMBL/GenBank data-base

mKO2-hCdt1(30/120)
mAG-hGeminin(1/110)
mKO2-zCdt1(1/190)
mAG-zGeminin(1/100)
mAG-hGeminin(1/60)
mCherry-hCdt1(30/120)
mVenus-hGeminin(1/110) 
mCherry-zCdt1(1/190)
mVenus-zGeminin(1/100) 
AmCyan-hGeminin(1/110)

AB370332
AB370333
AB505859
AB505860
AB505861
AB512478
AB512479

AB971706
AB971707
AB982071

Fucci technology

  • Sakaue-Sawano A et al.: Cell, 132: 487-498, 2008
  • Sakaue-Sawano A et al.: Chem. Biol., 15: 1243-1248, 2008
  • Sakaue-Sawano A & Miyawaki A: Imaging in Developmental Biology, 331-340, CSH Press, 2011
  • Nishimura K et al.: PLoS One, 8: e63001, 2013
  • Oki T et al.: Sci Rep, 4:4012, 2014

Fucci mouse

  • Sakaue-Sawano A et al.: Cell, 132:487-498, 2008
  • Aiba Y et al.: Proc. Natl. Acad. Sci. USA., 107: 12192-12197, 2010
  • Abe T et al.: Development, 140:237-246, 2013a
  • Sakaue-Sawano A et al.: Development, 140, 4624-4632, 2013b
  • Tomura M et al: PLoS One, 8: e73801, 2013
  • Mort RL et al.: Cell Cycle, 13:17, 2681-2696, 2014

Fucci zebrafish

  • Sugiyama M et al.: Proc. Natl. Acad. Sci. USA., 106: 20812-20817, 2009
  • Fukuhara S et al.:Dev Biol. ,393:10-23, 2014

Fucci ciona

  • Ogura Y et al.: Development, 138: 577-587, 2011

Fucci drosophila

  • Nakajima Y et al.: Mol. Cell Biol., 31: 2499-2512, 2011
  • Makhijani K et al.: Development., 138: 5379-5391, 2011

Application for stem cell research

  • Hama H et al.: Nat. Neuroscience, 14: 1481-1488, 2011
  • Yo M et al.: BBRC, 457, 7-11, 2014

Application for drug screening

  • Sakaue-Sawano A et al.: BMC Cell Biol., 12:2, 2011
  • Dan S et al.: Eur. J. Cancer. 48: 936-943, 2012

 

 

Last update: 05/06/2016