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lab head: Atsushi Miyawaki, M.D., Ph.D.

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Reference

Material

Paper

Xpa Tsutsui H, Jinno Y, Shoda K,Tomita A, Matsuda M,Yamashita E, Katayama H ,Nakagawa A,Miyawaki A. (2015) A Diffraction-Quality Protein Crystal Processed as an Autophagic Cargo, Mol. Cell, 58 (1), 186-193.
UnaG Kumagai A, Ando R, Miyatake H, Greimel P, Kobayashi T, Hirabayashi Y, Shimogori T, Miyawaki A. (2013) A Bilirubin-Inducible Fluorescent Protein from Eel Muscle. Cell, 153 (7): 1602-1611.
GEPRA Shimozono S, Iimura T, Kitaguchi T, Higashijima S, Miyawaki A. (2013) Visualization of an endogenous retinoic acid gradient across embryonic development, Nature, 496: 363-366.

Scale

Hama H, Kurokawa H, Kawano H, Ando R, Shimogori T, Noda H, Fukami K, Sakaue-Sawano A, Miyawaki A. (2011) Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain, Nature Neuroscience, 14 (11): 1481-1488.

Mermaid

Tsutsui H, Karasawa S, Okamura Y, Miyawaki A. (2008) Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat Methods, 5: 683-685.

Tsutsui H, Higashijima SI, Miyawaki A, Okamura Y. (2010) Visualizing Voltage Dynamics in Zebrafish Heart. J Physiol. 588 (Pt 12): 2017-2021.

Tsutsui H, Jinno Y, Tomita A, Niino Y, Yamada Y, Mikoshiba K, Miyawaki A, Okamura Y. (2013) Improved detection of electrical activity with a voltage probe based on a voltage-sensing phosphatase. J Physiol. 591 (Pt 18): 4427-4437.

Fucci

Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. (2008) Visualizing Spatiotemporal Dynamics of Multicellular Cell Cycle Progression. Cell, 132: 487-498.

Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. (2008) Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology, 15: 1243-1248.

Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817 .

Aiba Y, Kometani K, Hamadate M, Moriyama S, Sakaue-Sawano A, Tomura M, Luche H, Fehling HJ, Casellas R, Kanagawa O, Miyawaki A, Kurosaki T. (2010) Preferential localization of IgG memory B cells adjacent to contracted germinal centers. Proc Natl Acad Sci U S A. 107 (27): 12192-12197.

Ogura Y, Sakaue-Sawano A, Nakagawa M, Satoh N, Miyawaki A, Sasakura Y. (2011) Coordination of mitosis and morphogenesis: role of a prolonged G2 phase during chordate neurulation. Development, 138 (3): 577-587.

Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12:2.

Nakajima YI, Kuranaga E, Sugimura K, Miyawaki A, Miura M. (2011)  Non-autonomous Apoptosis is Triggered by Local Cell Cycle Progression during Epithelial Replacement in Drosophila. Mol Cell Biol., 31 (12): 2499-2512.

Dan S, Okamura M, Mukai Y, Yoshimi H, Inoue Y, Hanyu A, Sakaue-Sawano A, Imamura T, Miyawaki A, Yamori T. (2011)ZSTK474, a specific phosphatidylinositol 3-kinase inhibitor, induces G1 arrest of the cell cycle in vivo. Eur. J. Cancer, 48: 936-943.

Abe T, Sakaue-Sawano A, Kiyonari H, Shioi G, Inoue K, Horiuchi T, Nakao K, Miyawaki A, Aizawa S, Fujimori T. (2012) Visualization of cell cycle in mouse embryos with Fucci2 reporter directed by Rosa26 promoter. Development, 140 (1): 237-246.

Nishimura K, Oki T, Kitaura J, Kuninaka S, Saya H, Sakaue-Sawano A, Miyawaki A, Kitamura T. (2013) Targets MgcRacGAP for Destruction in the Late M Phase. PLoS One, 8 (5): e63001.

Tomura M, Sakaue-Sawano A, Mori Y, Takase-Utsugi M, Hata A, Ohtawa K, Kanagawa O, Miyawaki A. (2013) Contrasting Quiescent G0 Phase with Mitotic Cell Cycling in the Mouse Immune System. PLoS ONE 8(9): e73801.

pBS-coupler

Shimozono S, Miyawaki A. (2008) Engineering FRET Constructs Using CFP and YFP Methods Cell Biol. 85C: 381-393.

pCathB-zFP506

Katayama H, Yamamoto A, Yoshimori T, Mizushima N, Miyawaki A. (2008) GFP-like proteins stably accumulation in lysosomes, Cell Struct. Funct., 33: 1-12.

Keima

Kogure T, Karasawa S, Araki T, Saito K, Kinjo M, Miyawaki A. (2006) A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy Nature Biotechnology 24: 577-581.

Kawano H, Kogure T, Abe Y, Mizuno H, Miyawaki A. (2008) Two-photon dual-color imaging using fluorescent proteins. Nat Methods. 5(5): 373-374

Kogure T, Kawano H, Abe Y, Miyawaki A. (2008) Fluorescence imaging using a fluorescent protein with a large Stokes shift. Methods, 45: 223-226.

Cy11.5

Shimozono S; Hosoi H; Mizuno H; Fukano T; Tahara T; Miyawaki A. (2006) Concatenation of Cyan and Yellow Fluorescent Proteins for Efficient Resonance Energy Transfer. Biochemistry 45: 6267-6271.

KikGR

Tsutsui H, Karasawa S, Shimizu H, Nukina N, Miyawaki A. (2005) Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6: 233-238.

Tsutsui H, Shimizu H, Mizuno H, Nukina N, Furuta T, Miyawaki A. (2009) The E1 mechanism in photo-induced β-elimination reactions for green-to red conversion of fluorescent proteins. Chemistry & Biology, 16(11):1140-1147.

mKikGR Habuchi S, Tsutsui H, Kochaniak AB, Miyawaki A, van Oijen AM. (2008) mKikGR, a Monomeric Photoswitchable Fluorescent Protein. PloS ONE, 3(12):e3944.

KO, MiCy

Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381:307-312.

Dronpa

Ando R, Mizuno H, Miyawaki A. (2004) Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306: 1370-1373.

Ando R, Flors C, Mizuno H, Hofkens J, Miyawaki A, (2007) Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants, Biophysical J., 92: L97-99.

DRIP

Shimozono S, Fukano T, Kimura KD, Mori I, Kirino Y, Miyawaki A. (2004) Slow Ca2+ dynamics in pharyngeal muscles in Caenorhabditis elegans during fast pumping.EMBO Rep. 5: 521-526.

SCAT3.1

Nagai T, Miyawaki A. (2004) A high-throughput method for development of FRET-based indicators for proteolysis. Biochem. Biophys. Res. Commun. 319: 72-77.

AG

Karasawa S, Araki T, Yamamoto-Hino M, Miyawaki A. (2003) A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278: 34167-34171.

Kaede

Ando R, Hama H, Yamamoto-Hino M, Mizuno H, Miyawaki A. (2002) An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc. Natl. Acad. Sci. U S A. 99: 12651-12656.

Mizuno H, Mal TK, Tong KI, Ando R, Furuta T, Ikura M, Miyawaki A. (2003) Photo-induced peptide cleavage in the green-to-red conversion of a fluorescent protein. Mol. Cell. 12: 1051-1058.

Venus

Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20: 87-90.

Pericam

Nagai T, Sawano A, Park ES, Miyawaki A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U S A. 98: 3197-3202.

Miyawaki A. (2006) Ratiometric Pericam. Cell Biology 317-324.

Cameleon

Miyawaki A, Llopis J, Heim R, McCaffery JM, Adams JA, Ikura M, Tsien RY. (1997) Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin. Nature 388: 882-887.

Miyawaki A, Griesbeck O, Heim R, Tsien RY. (1999) Dynamic and quantitative Ca2+ measurements using improved cameleons. Proc. Natl. Acad. Sci. U S A. 96: 2135-2140.

Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559.

Mizuno H, Sassa T, Higashijima S, Okamoto H, Miyawaki A. (2013) Transgenic zebrafish for ratiometric imaging of cytosolic and mitochondrial Ca(2+) response in teleost embryo. Cell Calcium, 54 (3): 236-245.

Last update: 06/15/2015